短胜肽及其組合物用於治療或/ 及預防骨骼肌萎縮症或與其相關疾病之用途
PP622在骨骼肌細胞中具有細胞保護效應。
對C2C12細胞進行的PP622和DI-10處理顯示出劑量依賴性活化的Akt/mtor訊號通路，該通路參與了骨骼肌成肌過程。
根據Western blotting分析，PP622和DI-10誘導了Akt、mTOR蛋白的磷酸化，並在分化培養基中誘導了C2C12肌原細胞的肌肉分化。
在處理PP622和DI-10的細胞中，肌肉分化轉錄因子Foxo3A的磷酸化也增加了。此外，高葡萄糖引起的細胞活力降低在不同劑量高葡萄糖處理時也降低了。
此外，在分化培養基中，高葡萄糖挑戰導致的肌原纖維數量減少，但PP622處理增加了分化的肌原纖維數量。
進一步，在高葡萄糖挑戰下，AMPK和粒線體相關轉錄因子如PGC-1α的磷酸化被抑制，但PP622和DI-10處理恢復了蛋白質量。
我們首次證明了一種特定的馬鈴薯勝肽對肌肉萎縮具有治療效果。
此外，DI-10和PP622改善了肌肉分化和粒線體生物發生，並進一步提高了肌肉蛋白質並抑制了C212細胞在高葡萄糖條件下的肌肉蛋白質降解。
閱讀詳細資料
將胜肽用於製備預防或治療心臟相關疾病之醫藥組合物之用途及含有該胜肽之營養補充品 Peptide with prevention and treatment of cardiovascular diseases"
本發明所揭一種單離之胜肽，其胺基酸序列係編碼為SEQ ID No.1。該單離之胜肽係能夠抑制與心臟肥大路徑相關之訊息傳遞蛋白表現、抑制與心肌細胞凋亡相關蛋白之表現、抑制心臟纖維化相關蛋白之表現，而具有避免引發如心肌細胞凋亡、心臟纖維化、心臟肥大、心臟發炎或其他心臟病變之功效。據此，本發明所揭胜肽係能作為醫藥組合物之活性成份，達到預防或治療心血管疾病之功效。
閱讀詳細資料輸液容器之部分
【物品用途】
本設計係有關於一種醫療器材，其用於供受靜脈連續注射之受注射者穿戴於頸肩部位上。
【設計說明】
請參閱圖式，整體來說，本設計係具有一U形本體，其係由一撓性材料所製成，該本體具有一容室，用以容納液體，一穿戴空間，得用以對應受注射者之肩頸部位，二側部，分別位於該穿戴空間兩側，用以使本設計得以穩定地被穿戴於受注射者之肩頸部位上。更進一步來說，各二該側部相互對稱，並且，其表面係分別以弧面設計加以修飾，呈現一種飽滿滑順之外觀，更能提供使用者舒適好用之視覺享受。
換言之，本設計所提供之輸液容器不僅具有獨特新穎之造形設計，更兼備實用性與美觀度，獨具創意，也使產品本身能夠更符合使用者需求，不僅達到吸引消費者目光，也能增進使用上的舒服度。
牛樟芝之培養方法
本发明所述牛樟芝的培养方法，主要是通过光照波长改变，而使所培养出的牛樟芝的内活性成份含与种类量增加，并再通过调整如培养基含水量、培养基的组成成份及其比例、培养温度等环境因子，更得有助于提高牛樟芝内活性成份的含量与种类。
The invention discloses a culture method for Antrodia cinnamomea to elevate the amounts and types of the bioactive components in Antrodia cinnamomea through controlling the wavelength and intensity of the light. Furthermore, the modification of the environmental factors such as water content, composition and relative amount of the culture medium, and the culture temperature are capable of increasing the amounts and types of the bioactive components in Antrodia cinnamomea.
篩選融合細胞之方法
This present invention relates to a screening method for rapidly identifying the hybridomas upon cell size and the expression of an exogenous label such as fluorescence labeling. This method of this present invention can largely shortens the time cost for antibody development by saving the time period with comparison of traditional methods using cell culture with media such as HAT medium.
閱讀詳細資料牛樟芝之培養方法
The invention discloses a culture method for Antrodia cinnamomea to elevate the amounts and types of the bioactive components in Antrodia cinnamomea through controlling the wavelength and intensity of the light. Furthermore, the modification of the environmental factors such as water content, composition and relative amount of the culture medium, and the culture temperature are capable of increasing the amounts and types of the bioactive components in Antrodia cinnamomea.
閱讀詳細資料應用於分解有機酸的白地霉分離株 Application of Galactomyces geotrichum THUFS on fermentation of fruits and vegetables
從泡菜中分離的Galactomyces geotrichum THUFS(BCRC920079) 能分解不良氣味的有機酸如: Butanoic acid,Isobutyric acid, 2-Methylbutanoic acid ,Hexanoic acid ,Heptanoic acid 及Octanoic acid；並能產生具有水果香氣的化合物如:Isoamyl alcohol, Isoprensol, phenethyl alcohol。因此利用此菌株發酵可去除蔬果產品中因上述有機酸造成的不良氣味，並賦於天然的香氣。例如改善諾麗(noni)果汁不良風味，目前諾麗果汁是高價位的保健食品，每公升純果汁價格高達NT$1500以上。由於諾麗純果汁含有上述有機酸，為了降低不良氣味，市售產品通常摻配葡萄汁或其他果汁掩蓋之。摻配會稀釋諾麗純果汁的有效保健成分，並影響原汁的風味。利用此菌株發酵將可改善諾麗果汁風味，提升經濟效益。
閱讀詳細資料石斛多酚於保肝、治療/預防糖尿病之用途 Method for treating obesity
The present invention provides methods of administering a Dendrobium polyphenol in an amount effective to lower blood sugar, treat hepatic disease, obesity or diabetes. Exemplary hepatic disease includes fibrosis, fatty liver and hepatitis. Moreover, the Dendrobium polyphenol is extracted from the plants of the genus Dendrobium.
閱讀詳細資料牛樟芝之培養方法
【課題】本発明は,牛樟芝の有効成分含有量および種類量を増加させる牛樟芝の培養方法を提供する. 【解決手段】該牛樟芝の培養方法では,主に照射光波長を変えることにより,培養した牛樟芝の有効成分含有量および種類量を増加し,さらに培地含水量,培地の組成成分およびその比率,培養温度などの環境要因を調整することにより,牛樟芝の有効成分含有量および種類の増加に寄与する. 【選択図】図1
PROBLEM TO BE SOLVED: To provide a culturing method of antrodia cinnamomea in which active ingredient content and types of antrodia cinnamomea are increased.SOLUTION: In a culturing method of the antrodia cinnamomea, active ingredient content and types of cultured antrodia cinnamomea are increased by changing mainly an irradiation light wavelength, and furthermore, adjusting water content of culture medium, composition components of culture medium and their ratio, environmental factors, such as culture temperature, or the like contributes to increasing the effective component content and types of antrodia cinnamomea.
用以供培養牛樟芝之易採收培養裝置
本实用新型所提供用以供培养牛樟芝的易采收培养装置，其包含有一皿，具有一本体，一容室凹设于该本体中，并在该本体的一侧形成开口；一盖，可卸除地盖设于该本体上，用以将该容室的开口予以封闭，俾以使该容室与大气隔离；一透气部，设于该盖上，用以于该容室与大气间形成透气通道，使呈气态的物质可以经由该透气通道而在该容室与大气间移动。
The utility model provides a culturing device used for culturing antrodia camphorate and enabling harvesting of the antrodia camphorate to be easy. The culturing device comprises a vessel, a cover and an air-permeable part, wherein the vessel is provided with a body, a containing chamber is formed in the body in a concaved mode, an opening is formed in one side of the body, the cover is detachably arranged on the body in a covering mode and used for sealing the opening of the containing chamber, so that the containing chamber and the atmosphere are isolated, the air-permeable part is arranged on the cover so that an air-permeable passage can be formed between the containing chamber and the atmosphere, and a gaseous substance can pass through the air-permeable passage and move between the containing chamber and the atmosphere.
促脂解大豆胜肽之最適水解條件、序列及其應用 Optimal hydolysis conditions of soy protein to produce peptides with lipolysis-stimulating activity and their sequencing and use thereof
本發明所揭一種製備促脂解大豆蛋白水解物之方法，首先係先將一配置好預定濃度之分離大豆蛋白，再加入一風味蛋白酶(Flavourzyme)於一最適水解條件下，進行水解反應，其中，該分離大豆蛋白與風味蛋白酶之比例為100：1，而該最適水解條件係如下：反應酸鹼值介於7~7.5間、反應溫度為40~50℃、水解時間為100~150分鐘，得到一具有極佳之促脂解活性之大豆蛋白水解物。本發明係更進一步分離鑑定出該大豆蛋白水解物中之活性胜肽序列具有九種組合，分別為Val-His-Val-Val、Leu-Leu-Leu、Leu-Leu-Ile、Leu-Ile-Leu、Leu-Ile-Ile、Ile-Leu-Leu、Ile-Leu-Ile、Ile-Ile-Leu及Ile-Ile-Ile。
閱讀詳細資料食品調味裝置
本發明係一飲料調味蓋，係能蓋設於任一寶特瓶之瓶口，該蓋內部容置有可食用之調味品，經過一特定時間後，該調味品能從該蓋內部進入寶特瓶中，用以增添寶特瓶中飲料的風味，達到方便依照個人喜好調製飲料與調整濃度之目的。
閱讀詳細資料北蟲草黑豆發酵水萃物用於抗疲勞及提升運動效果之用途
北蟲草(Cordyceps militaris)是種珍貴藥用菇類，富含多種機能性成份，包括腺苷和蟲草素等。本專利技術主要將北蟲草接種於豆類基質，進行固態培養，所得固態發酵產品以水萃而得萃取物，並利用ICR小鼠模型進行抗疲勞效果評估。
動物實驗分為控制組(去離子水)、正控制組(長庚生物科技北蟲草菌絲體膠囊)及萃取物實驗組經28天餵食，萃取物分為高(1 g/kg)、中(0.5 g/kg)和低(0.25 g/kg)劑量3組，利用前肢抓力及游泳試驗，並分析小鼠血液中抗疲勞相關生化參數，測試北蟲草豆類萃取物是否具有抗疲勞效果。
研究結果顯示，在前肢抓力試驗方面，中(122.4 KgF)和高(133.6 KgF)萃取物劑量組顯著優於控制組(89.6 KgF)；另游泳試驗方面，高劑量組(870.2 s)顯著優於控制組(484.0 s)。此外，高劑量組可降低尿素氮、肌酸激酶、乳酸和乳酸去氫酶，另可透過調節肝醣及血糖提供能量來源。綜上可知北蟲草豆類固態發酵培養萃取物高劑量組(1 g/kg)具有抗疲勞效果。
閱讀詳細資料短胜肽及其組合物用於治療或/及預防骨骼肌萎縮症或與其相關疾病之用途
Sarcopenia is an aging associated involving skeletal muscle atrophy and reduction in muscle strength; and there are no pharmaceutical interventions available so far. Moreover, conditions like hyperglycaemia are known to further intensify muscle degradation. Therefore, novel strategies to attenuate skeletal muscle loss are essential to enhance muscle function and thereby to improve the quality of life in diabetic individuals. In this study we have investigated the efficiency of a soybean peptide fraction APPH and a short peptide from PP622 named DI-10 for their cytoprotective effects in skeletal muscle cells. PP622 and DI-10 treatment in C2C12 cells showed dose dependent activation of Akt/mtor signalling pathway that is involved in skeletal myogenesis. According to Western blotting analysis, PP622 and DI-10 induced the phosphorylation of Akt, mTOR proteins and induced myogenic differentiation of C2C12 myoblasts in a differentiation medium. The phosphorylation myogenic transcription factor Foxo3A was also found to be increased in the cells treated with PP622 and DI-10. In addition, reduction in cell viability caused by high glucose was also found to be reduced when treated with different doses of high glucose in a dose dependent manner. Moreover, number of myotybules in a differentiation medium reduced upon high glucose challenge, but treatment with PP622 increased the number of differentiated myotubules. Further, the phosphorylations of AMPK and mitochondrial-related transcription factors such as PGC-1α were suppressed upon high glucose challenge but PP622 and DI-10 treatment restored the protein levels. We demonstrate for the first time that a specific potato peptide has a therapeutic effect against sarcopenia. In addition, DI-10 and PP622 improved the myogenic differentiation and their mitochondrial biogenesis and further improved mygenic protein and inhibited muscle protein degradation in C212 cells challenged under high glucose condition.
肌少症是與老化相關的疾病，涉及骨骼肌萎縮和肌力減少；目前尚無有效的藥物治療方法。此外，像是高血糖等情況已知會進一步加劇肌肉退化。因此，開發新的策略來減緩骨骼肌損失對於提高肌肉功能並改善糖尿病患者的生活品質至關重要。在本研究中，我們探討了大豆肽分子APPH和PP622中短肽DI-10在骨骼肌細胞中的細胞保護效應。PP622和DI-10在C2C12細胞中的處理顯示出劑量依賴性地激活了涉及骨骼肌生成的Akt/mTOR信號通路。根據Western blot分析，PP622和DI-10誘導了Akt、mTOR蛋白的磷酸化，並在分化培養基中促進了C2C12肌母細胞的肌源性分化。在處理過PP622和DI-10的細胞中，我們還發現肌源性轉錄因子Foxo3A的磷酸化水準也有所增加。此外，在高糖挑戰下，細胞活力的下降被發現隨著不同劑量的高糖處理呈劑量依賴性減少。更重要的是，在分化培養基中，經高糖挑戰後，肌管數量減少，但PP622處理後，分化肌管的數量有所增加。此外，AMPK的磷酸化及與線粒體相關的轉錄因子如PGC-1α在高糖挑戰下被抑制，但PP622和DI-10的處理恢復了這些蛋白質的水平。我們首次證明了特定的馬鈴薯肽對抗肌少症的治療效果。此外，DI-10和PP622改善了肌源性分化、線粒體生物生成，並進一步改善了肌源性蛋白質，抑制了在高糖環境下C2C12細胞中的肌肉蛋白降解。
閱讀詳細資料